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polyclonal anti gusb ab  (Proteintech)


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    Structured Review

    Proteintech polyclonal anti gusb ab
    (A and B) Identification of Dectin-2-binding molecules on BMDCs. Affinity-purified proteins using Ig, Dectin-2 QPD -Ig (QPD) and Dectin-2-Ig (WT) were eluted with (A) heating or (B) addition of elution buffer containing 100mM Mannose. Top 5 proteins with high Mascot score were listed on the right side of the pictures. (C, D) <t>Gusb</t> expressions in BMDCs. Affinity-precipitated fractions with Ig and Dectin-2-Ig (D2) from whole cell lysate (WCL) of BMDCs (C) or culture supernatants (D) were analyzed by western blotting using anti-Gusb or anti-hIg mAb. (E) Reporter cells expressing FcRγ together with Dectin-2 were incubated with indicated cell numbers of Gusb-overexpressing RAW264.7 cells (black circle) or parental cells (Grey circle). (F) Dectin-2 reporter cells were incubated with Gusb-overexpressing RAW264.7 cells in the presence of mannose (100, 80, 60, 40 mM from the right) for 18 h. These data are presented as the means ± S.D. for duplicate assays, and representative of at least two independent experiments.
    Polyclonal Anti Gusb Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+anti+gusb+ab/pmc05207712-31-0-5?v=Proteintech
    Average 93 stars, based on 6 article reviews
    polyclonal anti gusb ab - by Bioz Stars, 2026-07
    93/100 stars

    Images

    1) Product Images from "C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells"

    Article Title: C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0169562

    (A and B) Identification of Dectin-2-binding molecules on BMDCs. Affinity-purified proteins using Ig, Dectin-2 QPD -Ig (QPD) and Dectin-2-Ig (WT) were eluted with (A) heating or (B) addition of elution buffer containing 100mM Mannose. Top 5 proteins with high Mascot score were listed on the right side of the pictures. (C, D) Gusb expressions in BMDCs. Affinity-precipitated fractions with Ig and Dectin-2-Ig (D2) from whole cell lysate (WCL) of BMDCs (C) or culture supernatants (D) were analyzed by western blotting using anti-Gusb or anti-hIg mAb. (E) Reporter cells expressing FcRγ together with Dectin-2 were incubated with indicated cell numbers of Gusb-overexpressing RAW264.7 cells (black circle) or parental cells (Grey circle). (F) Dectin-2 reporter cells were incubated with Gusb-overexpressing RAW264.7 cells in the presence of mannose (100, 80, 60, 40 mM from the right) for 18 h. These data are presented as the means ± S.D. for duplicate assays, and representative of at least two independent experiments.
    Figure Legend Snippet: (A and B) Identification of Dectin-2-binding molecules on BMDCs. Affinity-purified proteins using Ig, Dectin-2 QPD -Ig (QPD) and Dectin-2-Ig (WT) were eluted with (A) heating or (B) addition of elution buffer containing 100mM Mannose. Top 5 proteins with high Mascot score were listed on the right side of the pictures. (C, D) Gusb expressions in BMDCs. Affinity-precipitated fractions with Ig and Dectin-2-Ig (D2) from whole cell lysate (WCL) of BMDCs (C) or culture supernatants (D) were analyzed by western blotting using anti-Gusb or anti-hIg mAb. (E) Reporter cells expressing FcRγ together with Dectin-2 were incubated with indicated cell numbers of Gusb-overexpressing RAW264.7 cells (black circle) or parental cells (Grey circle). (F) Dectin-2 reporter cells were incubated with Gusb-overexpressing RAW264.7 cells in the presence of mannose (100, 80, 60, 40 mM from the right) for 18 h. These data are presented as the means ± S.D. for duplicate assays, and representative of at least two independent experiments.

    Techniques Used: Binding Assay, Affinity Purification, Western Blot, Expressing, Incubation

    (A) Schematic diagram of N-glycosylation sites on Gusb. (B) The importance of glycosylation for binding of Dectin-2. Flag-tagged Gusb were immunoprecipitated with biotinylated anti-Flag (M2) antibody followed by enrichment with streptavidin beads. After incubation of Flag-tagged Gusb proteins with Dectin-2-Ig, co-precipitated Dectin-2-Ig was detected by western blotting using anti-hIg mAb.
    Figure Legend Snippet: (A) Schematic diagram of N-glycosylation sites on Gusb. (B) The importance of glycosylation for binding of Dectin-2. Flag-tagged Gusb were immunoprecipitated with biotinylated anti-Flag (M2) antibody followed by enrichment with streptavidin beads. After incubation of Flag-tagged Gusb proteins with Dectin-2-Ig, co-precipitated Dectin-2-Ig was detected by western blotting using anti-hIg mAb.

    Techniques Used: Glycoproteomics, Binding Assay, Immunoprecipitation, Incubation, Western Blot

    (A) Gusb –/– mice were established by CRISPR-Cas9 system. Expression of Gusb on BMDCs was analyzed by western blotting. (B) Dectin-2-reporter cells recognized Gusb-deficient BMDCs. Reporter cells expressing Dectin-2 together with FcRγ were incubated with WT (black bar) or Gusb-deficient (grey bar) BMDCs for 18 h.
    Figure Legend Snippet: (A) Gusb –/– mice were established by CRISPR-Cas9 system. Expression of Gusb on BMDCs was analyzed by western blotting. (B) Dectin-2-reporter cells recognized Gusb-deficient BMDCs. Reporter cells expressing Dectin-2 together with FcRγ were incubated with WT (black bar) or Gusb-deficient (grey bar) BMDCs for 18 h.

    Techniques Used: CRISPR, Expressing, Western Blot, Incubation



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    polyclonal anti gusb ab  (Proteintech)
    93
    Proteintech polyclonal anti gusb ab
    (A and B) Identification of Dectin-2-binding molecules on BMDCs. Affinity-purified proteins using Ig, Dectin-2 QPD -Ig (QPD) and Dectin-2-Ig (WT) were eluted with (A) heating or (B) addition of elution buffer containing 100mM Mannose. Top 5 proteins with high Mascot score were listed on the right side of the pictures. (C, D) <t>Gusb</t> expressions in BMDCs. Affinity-precipitated fractions with Ig and Dectin-2-Ig (D2) from whole cell lysate (WCL) of BMDCs (C) or culture supernatants (D) were analyzed by western blotting using anti-Gusb or anti-hIg mAb. (E) Reporter cells expressing FcRγ together with Dectin-2 were incubated with indicated cell numbers of Gusb-overexpressing RAW264.7 cells (black circle) or parental cells (Grey circle). (F) Dectin-2 reporter cells were incubated with Gusb-overexpressing RAW264.7 cells in the presence of mannose (100, 80, 60, 40 mM from the right) for 18 h. These data are presented as the means ± S.D. for duplicate assays, and representative of at least two independent experiments.
    Polyclonal Anti Gusb Ab, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+anti+gusb+ab/pmc05207712-31-0-5?v=Proteintech
    Average 93 stars, based on 1 article reviews
    polyclonal anti gusb ab - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    (A and B) Identification of Dectin-2-binding molecules on BMDCs. Affinity-purified proteins using Ig, Dectin-2 QPD -Ig (QPD) and Dectin-2-Ig (WT) were eluted with (A) heating or (B) addition of elution buffer containing 100mM Mannose. Top 5 proteins with high Mascot score were listed on the right side of the pictures. (C, D) Gusb expressions in BMDCs. Affinity-precipitated fractions with Ig and Dectin-2-Ig (D2) from whole cell lysate (WCL) of BMDCs (C) or culture supernatants (D) were analyzed by western blotting using anti-Gusb or anti-hIg mAb. (E) Reporter cells expressing FcRγ together with Dectin-2 were incubated with indicated cell numbers of Gusb-overexpressing RAW264.7 cells (black circle) or parental cells (Grey circle). (F) Dectin-2 reporter cells were incubated with Gusb-overexpressing RAW264.7 cells in the presence of mannose (100, 80, 60, 40 mM from the right) for 18 h. These data are presented as the means ± S.D. for duplicate assays, and representative of at least two independent experiments.

    Journal: PLoS ONE

    Article Title: C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells

    doi: 10.1371/journal.pone.0169562

    Figure Lengend Snippet: (A and B) Identification of Dectin-2-binding molecules on BMDCs. Affinity-purified proteins using Ig, Dectin-2 QPD -Ig (QPD) and Dectin-2-Ig (WT) were eluted with (A) heating or (B) addition of elution buffer containing 100mM Mannose. Top 5 proteins with high Mascot score were listed on the right side of the pictures. (C, D) Gusb expressions in BMDCs. Affinity-precipitated fractions with Ig and Dectin-2-Ig (D2) from whole cell lysate (WCL) of BMDCs (C) or culture supernatants (D) were analyzed by western blotting using anti-Gusb or anti-hIg mAb. (E) Reporter cells expressing FcRγ together with Dectin-2 were incubated with indicated cell numbers of Gusb-overexpressing RAW264.7 cells (black circle) or parental cells (Grey circle). (F) Dectin-2 reporter cells were incubated with Gusb-overexpressing RAW264.7 cells in the presence of mannose (100, 80, 60, 40 mM from the right) for 18 h. These data are presented as the means ± S.D. for duplicate assays, and representative of at least two independent experiments.

    Article Snippet: Polyclonal anti-Gusb Ab was from proteintech.

    Techniques: Binding Assay, Affinity Purification, Western Blot, Expressing, Incubation

    (A) Schematic diagram of N-glycosylation sites on Gusb. (B) The importance of glycosylation for binding of Dectin-2. Flag-tagged Gusb were immunoprecipitated with biotinylated anti-Flag (M2) antibody followed by enrichment with streptavidin beads. After incubation of Flag-tagged Gusb proteins with Dectin-2-Ig, co-precipitated Dectin-2-Ig was detected by western blotting using anti-hIg mAb.

    Journal: PLoS ONE

    Article Title: C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells

    doi: 10.1371/journal.pone.0169562

    Figure Lengend Snippet: (A) Schematic diagram of N-glycosylation sites on Gusb. (B) The importance of glycosylation for binding of Dectin-2. Flag-tagged Gusb were immunoprecipitated with biotinylated anti-Flag (M2) antibody followed by enrichment with streptavidin beads. After incubation of Flag-tagged Gusb proteins with Dectin-2-Ig, co-precipitated Dectin-2-Ig was detected by western blotting using anti-hIg mAb.

    Article Snippet: Polyclonal anti-Gusb Ab was from proteintech.

    Techniques: Glycoproteomics, Binding Assay, Immunoprecipitation, Incubation, Western Blot

    (A) Gusb –/– mice were established by CRISPR-Cas9 system. Expression of Gusb on BMDCs was analyzed by western blotting. (B) Dectin-2-reporter cells recognized Gusb-deficient BMDCs. Reporter cells expressing Dectin-2 together with FcRγ were incubated with WT (black bar) or Gusb-deficient (grey bar) BMDCs for 18 h.

    Journal: PLoS ONE

    Article Title: C-Type Lectin Receptor Dectin-2 Binds to an Endogenous Protein β-Glucuronidase on Dendritic Cells

    doi: 10.1371/journal.pone.0169562

    Figure Lengend Snippet: (A) Gusb –/– mice were established by CRISPR-Cas9 system. Expression of Gusb on BMDCs was analyzed by western blotting. (B) Dectin-2-reporter cells recognized Gusb-deficient BMDCs. Reporter cells expressing Dectin-2 together with FcRγ were incubated with WT (black bar) or Gusb-deficient (grey bar) BMDCs for 18 h.

    Article Snippet: Polyclonal anti-Gusb Ab was from proteintech.

    Techniques: CRISPR, Expressing, Western Blot, Incubation